Human eIF-5A1/EIF5A antibody | NKMAX ATGA0128 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human eIF-5A1/EIF5A antibody

catalog :

ATGA0128

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4D1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human eIF-5A1/EIF5A antibody

Catalog number (SKU) :

ATGA0128

NCBI :

NP_001961

Description :

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.

Alternative Names :

Eukaryotic translation initiation factor 5A-1 isoform B, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Saini P., et al. (2009) Nature. 459(7243): 118-21.;; Gosslau A., et al. (2009) J Cell Physiol. 219(2): 485-93.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human EIF5A (1-154aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of EIF5A in Balb/3T3 cells. The cell was stained with ATGA0128 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF5A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: 293T cell lysate;;Lane 3.: HeLa cell lysate;;Lane 4.: MCF7 cell lysate;;Lane 5.: NIH-3T3 cell lysate

Reacts With :

Human

Clone Number :

AT4D1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P63241

Internal Search Terms :

eIF5A,ATGA0128-10ug,ATGA0128-20ug,ATGA0128-50ug,ATGA0128-100ug,ATGA0128-250ug,ATGA0128-500ug,ATGA0128-1mg,ATGA0128-10,ATGA0128-20,ATGA0128-50,ATGA0128-100,ATGA0128-250,ATGA0128-500,ATGA0128-1

more info or order :

NKMAX product webpage