Human ADRM1 antibody | NKMAX ATGA0127 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ADRM1 antibody

catalog :

ATGA0127

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT34C2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ADRM1 antibody

Catalog number (SKU) :

ATGA0127

NCBI :

NP_783163

Description :

ADRM1, also known as proteasomal ubiquitin receptor ADRM1, functions as a proteasomal ubiquitin receptor. This protein is an integral plasma membrane protein which promotes cell adhesion. ADRM1 is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells.

Alternative Names :

Proteasomal ubiquitin receptor ADRM1, ARM1, GP110, Rpn13

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hamazaki J., et al. (2006) EMBO J. 25: 4524-4536. ;; Qiu X.-B., et al. (2006) EMBO J. 25: 5742-5753.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human ADRM1 (1-407aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of ADRM1 in MCF7 cells. The cell was stained with ATGA0127 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADRM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MCF7 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: K562 cell lysate;;Lane 4.: PC3 cell lysate;;Lane 5.: 293T cell lysate;;Lane 6.: HepG2 cell lysate;;Lane 7.: Jurkat cell lysate;;Lane 8.: U87MG cell lysate

Reacts With :

Human

Clone Number :

AT34C2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ADRM1 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q16186

Internal Search Terms :

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more info or order :

NKMAX product webpage