Human OSTF1 antibody | NKMAX ATGA0125 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human OSTF1 antibody

catalog :

ATGA0125

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT9G4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human OSTF1 antibody

Catalog number (SKU) :

ATGA0125

NCBI :

AAH07459

Description :

Osteoclast-stimulating factor (OSTF1) is an intracellular signaling protein, highly expressed by osteoclasts (OCL). Indirectly, it enhances osteoclast (OCL) formation and bone resorption through a cellular signal transduction cascade by possibly interacting with c-Src or other c-Src-related proteins. OSTF1 contains a short proline-rich N-terminal region, an SH3 domain, three ankyrin repeats and an aspartate-rich C-terminal region, suggesting that it is potentially involved in protein-protein interactions.

Alternative Names :

Osteoclast stimulating factor 1, SH3P2, OSF, Osteoclast stimulating factor 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kurihara N., et al. (2001) J. Biol. Chem. 276(44): 41035-9. ;; Reddy S., et al. (1999) J Cell Physiol. 177(4): 636-45.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human OSTF1 (1-217aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of OSTF1 in MCF7 cells. The cell was stained with ATGA0125 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of OSTF1 in A431 cells. The cell was stained with ATGA0125 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0125-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: MCF7 cell lysate;;Lane 3.: Jurkat cell lysate;;Lane 4.: LnCap cell lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: OSTF1 Transfected 293T cell lysate {ATGA0125-WB2.jpg}

Reacts With :

Human

Clone Number :

AT9G4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q92882

Internal Search Terms :

OSTF1,ATGA0125-10ug,ATGA0125-20ug,ATGA0125-50ug,ATGA0125-100ug,ATGA0125-250ug,ATGA0125-500ug,ATGA0125-1mg,ATGA0125-10,ATGA0125-20,ATGA0125-50,ATGA0125-100,ATGA0125-250,ATGA0125-500,ATGA0125-1

more info or order :

NKMAX product webpage