Human PEDFR/PNPLA2 antibody | NKMAX ATGA0122 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PEDFR/PNPLA2 antibody

catalog :

ATGA0122

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT18E6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PEDFR/PNPLA2 antibody

Catalog number (SKU) :

ATGA0122

NCBI :

NP_065109

Description :

Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.

Alternative Names :

Adipose triglyceride lipase, Calcium-independent phospholipase A2, Desnutrin IPLA2-zeta, Pigment epithelium-derived factor receptor, TTS2.2, Transport-secretion protein 2, TTS2, ATGL, Patatin-like phospholipase domain-containing protein 2, PNPLA2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Fischer J, et al., (2007) Nat Genet. 39(1): 28-30.;; Elena S, et al., (2006) EMBO Rep. 7(1): 106-113.;; Zimmermann R, et al., (2004) Science. 306(5700): 1383-1386.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human ATGL (30-504aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of ATGL in Hep3B cells. The cell was stained with ATGA0122 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of ATGL in HeLa cells. The cell was stained with ATGA0122 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010103622287.jpg}ICC/IF analysis of ATGL in A549 cells. The cell was stained with ATGA0122 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010103476044.jpg} {ATGA0122-IF3b.jpg}

WB Comment and Pic :

The A431 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The mouse adipose tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0122-WB2.jpg}

Reacts With :

Human

Clone Number :

AT18E6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with ATGA0122 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with ATGA0122 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).{2004010103833302.jpg}

Antigen Species :

Human

Gene Category :

Cardiovascular

Additional Comment Only :

ATGA0135 has been replaced with a catalog number ATGA0122.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q96AD5

Internal Search Terms :

TTS2.2, TTS2, Transport-secretion protein 2, PNPLA2, Pigment epithelium-derived factor receptor, PEDF PNPLA2, Patatin-like phospholipase domain-containing protein 2, DesnutrinIPLA2-zeta, Desnutrin IPLA2-zeta, Calcium-independent phospholipase A2, ATGL, Adipose triglyceride lipase, ATGA0122-10ug, ATGA0122-20ug, ATGA0122-50ug, ATGA0122-100ug, ATGA0122-250ug, ATGA0122-500ug, ATGA0122-1mg, ATGA0122-010, ATGA0122-020, ATGA0122-050, ATGA0122-100, ATGA0122-250, ATGA0122-500, ATGA0122-01M

more info or order :

NKMAX product webpage