Human TDP-43/TARDBP antibody | NKMAX ATA0834 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human TDP-43/TARDBP antibody

catalog :

ATA0834

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1B8

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human TDP-43/TARDBP antibody

Catalog number (SKU) :

ATA0834

NCBI :

NP_031401

Description :

TAR DNA binding protein (TARDBP), also known as TDP-43, is a cellular protein. TARDBP was identified as the disease accumulating protein in patients with frontotemporal lobar degeneration (FTLD) with ubiquitin inclusions (FTLD-U) and in amyotrophic lateral sclerosis (ALS). TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV. Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription. In addition, this protein regulates alternate splicing of the cystic fibrosis transmembrane receptor (CFTR) gene.

Alternative Names :

TAR DNA binding protein, TDP-43, ALS10, TAR DNA-binding protein 43, TDP43

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Nicola JR, et al., (2008) PLoS Genet 4(9):e1000193.;; Kabashi E, et al., (2008) Nat Genet 40(5):572-574.;;Ou SH, et al., (1995) J Virol 69:3584-3596. ;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human TARDBP (1-260aa) purified from E. coli

Applications :

ELISA, WB, IHC, FACS

WB Comment and Pic :

The cell lysates (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TARDBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HeLa cell lysate;;

Reacts With :

Human

Clone Number :

k1B8

Ab Usage :

The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of TARDBP in HeLa cells. The cell was stained with ATA0834 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

IHC Comment And Pic :

Paraffin embedded sections of human prostate cancer tissue were incubated with anti-human TARDBP(1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Paraffin embedded sections of human seminoma tissue were incubated with anti-human TARDBP(1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {ATA0834-IHC1b.jpg}

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q13148

Internal Search Terms :

TDP43, TARDBP,ATA0834-10ug,ATA0834-20ug,ATA0834-50ug,ATA0834-100ug,ATA0834-250ug,ATA0834-500ug,ATA0834-1mg,ATA0834-10,ATA0834-20,ATA0834-50,ATA0834-100,ATA0834-250,ATA0834-500,ATA0834-1

more info or order :

NKMAX product webpage