Human GRP94/HSP90B1 antibody | NKMAX ATA0623 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GRP94/HSP90B1 antibody

catalog :

ATA0623

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2H3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GRP94/HSP90B1 antibody

Catalog number (SKU) :

ATA0623

NCBI :

NP_003290

Description :

Tumor rejection antigen 1 (TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.

Alternative Names :

94 kDa glucose-regulated protein, 94 kDa glucose-regulated protein, HSP90B1, GP96, TRA1, Tumor rejection antigen 1, GRP94, 94 kDa glucose-regulated protein 94 kDa glucose regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 9, Heat shock protein 90 kDa beta member 1, Stress inducible tumor rejection antigen GP96, Tumor rejection antigen gp96.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wulf GM, et al., (2002) J Biol. Chem. 277(50):47976-47979.;; Hamdane M, et al., (2002) J Mol Neurosci. 19(3):275-287.;; Zheng H, et al., (2002) Nature 419(6909):853-857.;; Lu,k.P. et al., (1996) Nature 380(6574):544-547.;; Campbell,H.D, et al., (1997) Genomics 44(2):157-162.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human TRA1 (676-803aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)

WB Comment and Pic :

The cell lysates of hES(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA1 (1:500). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : ES cell lysate

Reacts With :

Human

Clone Number :

2H3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Heat Shock Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P14625

Internal Search Terms :

gp96, HSP90B1, TRA1,ATA0623-10ug,ATA0623-20ug,ATA0623-50ug,ATA0623-100ug,ATA0623-250ug,ATA0623-500ug,ATA0623-1mg,ATA0623-10,ATA0623-20,ATA0623-50,ATA0623-100,ATA0623-250,ATA0623-500,ATA0623-1

more info or order :

NKMAX product webpage