Human STEAP1 antibody | NKMAX AST0910 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human STEAP1 antibody

catalog :

AST0910

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J2D2

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human STEAP1 antibody

Catalog number (SKU) :

AST0910

NCBI :

NP_036581

Description :

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewings sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.

Alternative Names :

six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

David A. Rodeberg, et al. (2005) Clin Cancer Res. June 11(12): 4545-4552.;; Pia M. Challita-Eid, et al., (2007) Cancer research June 67(12): 5798-5805.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human STEAP1 (1-70aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC

IF Comment And Pic :

ICC/IF analysis of STEAP1 in LNCap cells. The cell was stained with AST0910 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(30ug) and tissue lysates(30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: LNcap cell lysate;; Lane 2.: Mouse Testis Tissue lysate;;

Reacts With :

Human

Clone Number :

J2D2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AST0910-IHC1b.jpg}

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9UHE8

Internal Search Terms :

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more info or order :

NKMAX product webpage