Human Synuclein-alpha/beta antibody | NKMAX ASC0632 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Synuclein-alpha/beta antibody

catalog :

ASC0632

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3B6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Synuclein-alpha/beta antibody

Catalog number (SKU) :

ASC0632

NCBI :

NP_000336, NP_003076

Description :

alpha-synuclein (amino acids 119-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.

Alternative Names :

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jakes R., et al. (1994) FEBS lett. 345, 27-32.;; Ueda K., et al. (1993) Proc. Natl.Acad. Sci. USA 90, 11282-11286.;; Kim J. (1997) Molecules and Cells 7, 78-83.;; Paik S. R., et al. (1997) Arch. Biochem. Biophys. 344, 325-334.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human a-synuclein (119-140aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of alpha, beta-Synuclein in LNCaP cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). ICC/IF analysis of alpha, beta-Synuclein in U87MG cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ASC0632-IF2.jpg}ICC/IF analysis of alpha, beta-Synuclein in C6 cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ASC0632-IF3.jpg}

WB Comment and Pic :

The recombinant protein (20ng) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human alpha-synuclein protein;;Lane 2.: Recombinant human beta-synuclein protein;;Lane 3.: Recombinant human gamma-synuclein protein;;Lane 4.: Mouse brain Tissue lysate;;Lane 5.: Rat brain Tissue lysate;;

Reacts With :

Human

Clone Number :

3B6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of alpha, beta-Synuclein in LNCaP cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Flow cytometry analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {ASC0632-FC2.jpg}Flow cytometry analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {ASC0632-FC3.jpg}

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P37840, Q16143

Internal Search Terms :

Synuclein beta, Synuclein alpha, SNCB protein, SNCB, SNCA protein, SNCA, PD1, Parkinson disease familial 1, PARK4, PARK1, Non A4 component of amyloid precursor, Non A beta component of AD amyloid, NACP, beta synuclein, alpha synuclein, a/b-Synuclein, a,b-Synuclein, a b-Synuclein, ASC0632-10ug, ASC0632-20ug, ASC0632-50ug, ASC0632-100ug, ASC0632-250ug, ASC0632-500ug, ASC0632-1mg, ASC0632-010, ASC0632-020, ASC0632-050, ASC0632-100, ASC0632-250, ASC0632-500, ASC0632-01M

more info or order :

NKMAX product webpage