Human RAB5A antibody | NKMAX ARA0803 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human RAB5A antibody

catalog :

ARA0803

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human RAB5A antibody

Catalog number (SKU) :

ARA0803

NCBI :

NP_004153

Description :

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

Alternative Names :

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bucci C, et al., (1992) Cell 70 (5): 715-28.;;Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1): 679-85.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Rab5a (1-215aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ARA0803 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysate of mouse brain (45ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

3A4

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P20339

Internal Search Terms :

Rab5a,ARA0803-10ug,ARA0803-20ug,ARA0803-50ug,ARA0803-100ug,ARA0803-250ug,ARA0803-500ug,ARA0803-1mg,ARA0803-10,ARA0803-20,ARA0803-50,ARA0803-100,ARA0803-250,ARA0803-500,ARA0803-1

References with this Product :

Del Cid-Pellitero E, et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. (Front Syst Neurosci. 2017){https://pubmed.ncbi.nlm.nih.gov/28408870/};;Winer H, et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. (Nat Commun. 2018){https://pubmed.ncbi.nlm.nih.gov/30218067/}

more info or order :

NKMAX product webpage