Human PP2C gamma/PPM1G antibody | NKMAX APP0902 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PP2C gamma/PPM1G antibody

catalog :

APP0902

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1G6

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PP2C gamma/PPM1G antibody

Catalog number (SKU) :

APP0902

NCBI :

NP_002698

Description :

PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Suh EJ., et al. (2006) FEBS Lett 580 (26):6100-6104.;; Marc Flajolet., et al. (2003) Proc Natl Acad Sci 100(26):16006-16011.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PPM1G (317-546aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC

IF Comment And Pic :

ICC/IF analysis of PPM1G in 293T cells. The cell was stained with APP0902 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The 293T cell lysate (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

k1G6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O15355

Internal Search Terms :

Protein phosphatase, Mg2+/Mn2+ dependent 1G, Protein phosphatase Mg2+/Mn2+ dependent 1G, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase magnesium dependent 1 gamma, Protein phosphatase 2C gamma isoform, Protein phosphatase 2 catalytic subunit gamma isoform, Protein phosphatase 1G, formerly 2C, magnesium-dependent, gamma isoform, Protein phosphatase 1G, Protein phosphatase 1C, PPP2CG, PPM1G, PPM1C, PP2Cgamma, PP2CG, PP2C gamma, PP2C g, PP2C, MGC2870, MGC1675, APP0902-10ug, APP0902-20ug, APP0902-50ug, APP0902-100ug, APP0902-250ug, APP0902-500ug, APP0902-1mg, APP0902-010, APP0902-020, APP0902-050, APP0902-100, APP0902-250, APP0902-500, APP0902-01M

more info or order :

NKMAX product webpage