Human CPI-17/PPP1R14A antibody | NKMAX APP0832 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CPI-17/PPP1R14A antibody

catalog :

APP0832

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4H10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CPI-17/PPP1R14A antibody

Catalog number (SKU) :

APP0832

NCBI :

NP_150281

Description :

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.

Alternative Names :

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hamaguchi., et al. (2000) Biochem. Biophys. Res Commun. 274(3):825-30.;; Eto M., et al. (1997) FEBS Lett. 410(2-3):356-60.;; Lartey I., et al. (2007) Biol Reprod. 76(6):971-82.;;

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PPP1R14A (1-147aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PPP1R14A in A549 cells. The cell was stained with APP0832 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : A549 cell lysate ;;Lane 2. : NIH-3T3 cell lysate ;;

Reacts With :

Human

Clone Number :

4H10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q96A00

Internal Search Terms :

Regulatory subunit 14A, Regulatory (inhibitor) subunit 14A CPI 17, Regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1, PPP1R14A, PPP1INL, PKC potentiated inhibitory protein of PP1, CPI-17, CPI17, CPI 17alpha, CPI 17 alpha, CPI-17 alpha, CPI17 alpha, APP0832-10ug, APP0832-20ug, APP0832-50ug, APP0832-100ug, APP0832-250ug, APP0832-500ug, APP0832-1mg, APP0832-010, APP0832-020, APP0832-050, APP0832-100, APP0832-250, APP0832-500, APP0832-01M

more info or order :

NKMAX product webpage