Human PP1 Catalytic Subunit/PPP1CA antibody | NKMAX APP0808 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PP1 Catalytic Subunit/PPP1CA antibody

catalog :

APP0808

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PP1 Catalytic Subunit/PPP1CA antibody

Catalog number (SKU) :

APP0808

NCBI :

NP_002699

Description :

Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis.

Alternative Names :

serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1, protein phosphatase 1 catalytic subunit alpha isozyme, PP-1A, PP1alpha, ppp1a, MGC15877, MGC1674

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cohen PT. (2002) J Cell Sci. 115(2):241-56.;;Hsu LC, et al. (2006) Oncogene. 25(40):5517-26.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PPP1CA (30-299aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PPP1CA in HeLa cells. The cell was stained with APP0808 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of HepG2 (45ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

4G3

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P62136

Internal Search Terms :

PP1 Catalytic Subunit, PPP1CA,APP0808-10ug,APP0808-20ug,APP0808-50ug,APP0808-100ug,APP0808-250ug,APP0808-500ug,APP0808-1mg,APP0808-10,APP0808-20,APP0808-50,APP0808-100,APP0808-250,APP0808-500,APP0808-1

more info or order :

NKMAX product webpage