Human Pin1 antibody | NKMAX API0622 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Pin1 antibody

catalog :

API0622

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Pin1 antibody

Catalog number (SKU) :

API0622

NCBI :

NP_006212

Description :

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLk I, Wee, and Myt1 kinases), several transcription factors likebeta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Alternative Names :

Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 5.2.1.8, Rotamase Pin1, PPIase Pin1, DOD, uBL5, PIN1, PPIase, EC 5.2.1.8, Rotamase Pin1, PPIase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wulf GM, et al., (2002) J Biol. Chem. 277(50):47976-47979.;; Hamdane M, et al., (2002) J Mol Neurosci. 19(3):275-287.;; Zheng H, et al., (2002) Nature 419(6909):853-857.;; Lu KP. et al., (1996) Nature 380(6574):544-547.;; Campbell HD, et al., (1997) Genomics 44(2):157-162

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Pin1 (1-163aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates(50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate;; Lane 2.: HeLa cell lysate;; Lane 3: Jurkat cell lysate;;

Reacts With :

Human

Clone Number :

3G8

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Pin1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q13526

Internal Search Terms :

Pin1,API0622-10ug,API0622-20ug,API0622-50ug,API0622-100ug,API0622-250ug,API0622-500ug,API0622-1mg,API0622-10,API0622-20,API0622-50,API0622-100,API0622-250,API0622-500,API0622-1

more info or order :

NKMAX product webpage