Human PGP9.5/UCHL1 antibody | NKMAX APG0714 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PGP9.5/UCHL1 antibody

catalog :

APG0714

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D9

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PGP9.5/UCHL1 antibody

Catalog number (SKU) :

APG0714

NCBI :

NP_004172

Description :

PGP9. 5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9. 5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9. 5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.

Alternative Names :

Ubiquitin C-terminal hydrolase L1, Ubiquitin carboxyl-terminal esterase L1, Ubiquitin thiolesterase L1, Parkinson Disease 5, PARK5, PGP9.5, Uch-L1, UCHL-1, Neuron cytoplasmic protein 9.5

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

kenji H et al , (1999) Am J Pathol 155(3):711-715.;;Liu Y et al , (2002) Cell 111(2):209-18.;;Mikako Sakurai.et al , (2006) Cell 119:162-171.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PGP9.5 (1-223aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC

IF Comment And Pic :

ICC/IF analysis of PGP9.5 in U87MG cells. The cell was stained with APG0714 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Brain tissue lysate;;Lane 2.: U87MG cell lysate

Reacts With :

Human

Clone Number :

3D9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P09936

Internal Search Terms :

UCH-L1, PGP9.5,APG0714-10ug,APG0714-20ug,APG0714-50ug,APG0714-100ug,APG0714-250ug,APG0714-500ug,APG0714-1mg,APG0714-10,APG0714-20,APG0714-50,APG0714-100,APG0714-250,APG0714-500,APG0714-1

more info or order :

NKMAX product webpage