Human Podoplanin antibody | NKMAX APD0833 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Podoplanin antibody

catalog :

APD0833

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5E2

reactivity :

human

application :

ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Podoplanin antibody

Catalog number (SKU) :

APD0833

NCBI :

NP_006465

Description :

Podoplanin (PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character.

Alternative Names :

TIA2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, Lung type I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein isoform a AGGRuS, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, GP40, Gp38, GP36, Glycoprotein 36 KD

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wicki A., et al. (2006) Br J Cancer. 96(1):1-5.;; Zimmer G., et al. (1999) Biochem J. 341(Pt2):277-84.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PDPN (1-206aa) purified from E. coli

Applications :

ELISA, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of Podoplanin in HaLe cells. The cell was stained with APD0833 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Reacts With :

Human

Clone Number :

5E2

Ab Usage :

The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with APD0833 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q86YL7

Internal Search Terms :

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more info or order :

NKMAX product webpage