Human PD-1 antibody | NKMAX APD0811 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PD-1 antibody

catalog :

APD0811

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4F12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PD-1 antibody

Catalog number (SKU) :

APD0811

NCBI :

AAH74740

Description :

PD-1 is an immunoreceptor that belongs to the immunoglobulin (Ig) superfamily, also known as Programmed Cell Death 1. It is expressed on activated T cell, B cells, myeloid cells and a subset of thymocytes. PD-1 interacts with expressed PDL1 and PDL2 to inhibit the proliferation and activation of T cells and B cells. Both PDL1 and PDL2 are expressed in a wide variety of normal tissues including heart, lung, placenta, spleen and kidney. PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases.

Alternative Names :

Programmed cell death 1, CD279, PD1, hPD-1, hPD-l, PDCD1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Freeman GJ, et al., (2000) J Exp Med. 192(7):1027-34.;;Latchman Y, et al., (2001) Nat Immunol. 2(3):261-8.;;Nishimura H, HonjoT., (2001) Trends Immunol. 22(5):265-8.;;Okazaki T, et al., (2001) Proc Natl Acad Sci USA. 98(24):13866-71.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PDCD1 (21-167aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of PD-1 in HeLa cells. The cell was stained with APD0811 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PD-1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: Raji cell lysate

Reacts With :

Human

Clone Number :

4F12

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PD-1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q15116

Internal Search Terms :

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more info or order :

NKMAX product webpage