Human NPM1 antibody | NKMAX ANP0626 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NPM1 antibody

catalog :

ANP0626

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5E3

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NPM1 antibody

Catalog number (SKU) :

ANP0626

NCBI :

NP_002511

Description :

Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.

Alternative Names :

nucleophosmin (nucleolar phosphoprotein B23,numatrin),B23, NPM, MGC104254, nucleolar phosphoprotein B23, numatrin, nucleophosmin/nucleoplasmin family, member 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Maiguel DA et al., (2004) Mol cell Biol 24:3703-3711.;; Colombo Emanuela et al., (2005) Mol cell Biol 25:8874-8886.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human NPM (81-294aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC, FACS

IF Comment And Pic :

ICC/IF analysis of NPM in HeLa cells. The cell was stained with ANP0626 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

5E3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NPM in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

IHC Comment And Pic :

This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P06748

Internal Search Terms :

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References with this Product :

Huang M, et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. (Oncotarget. 2016){https://pubmed.ncbi.nlm.nih.gov/27732946/}

more info or order :

NKMAX product webpage