Human alpha-Synuclein antibody | NKMAX ANC0603 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human alpha-Synuclein antibody

catalog :

ANC0603

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5C2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human alpha-Synuclein antibody

Catalog number (SKU) :

ANC0603

NCBI :

NP_000336

Description :

alpha-synuclein (amino acids 1-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques (NAC, amino acids 61-95) is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.

Alternative Names :

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jakes, R., et al. (1994) FEBS lett. 345:27-32.;; Ueda, K., et al. (1993) Proc. Natl.Acad. Sci. USA 90:11282-11286.;; Kim, J. (1997) Molecules and Cells 7:78-83.;; Paik, S. R., et al. (1997) Arch. Biochem. Biophys. 344:325-334.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human alpha-synuclein (61-95) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of alpha-Synuclein in HeLa cells. The cell was stained with ANC0603 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human alpha-synuclein protein;;Lane 2.: Recombinant human beta-synuclein protein;;Lane 3.: Recombinant human gamma-synuclein proteinThe recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein (61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human a-synuclein(1-60aa);;Lane 2.: Recombinant human a-synuclein(1-95aa);;Lane 3.: Recombinant human a-synuclein(61-140aa);;Lane 4.: Recombinant human a-synuclein(96-140aa){2004010016919061.jpg}

Reacts With :

Human

Clone Number :

5C2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P37840

Internal Search Terms :

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more info or order :

NKMAX product webpage