Human Nanog antibody | NKMAX ANA0837 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Nanog antibody

catalog :

ANA0837

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A10

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Nanog antibody

Catalog number (SKU) :

ANA0837

NCBI :

NP_079141

Description :

Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Alternative Names :

Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tay Y., et al. (2008) Nature. 455(7216):1124-8;; Chiou SH., et al. (2008) Clin Cancer Res. 14(13):4085-95;; Boer B., et al. (2008) Mol Reprod Dev.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human Nanog (1-154aa) purified from E. coli

Applications :

ELISA, WB, IHC, FACS

WB Comment and Pic :

The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

5A10

Ab Usage :

The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IHC Comment And Pic :

Paraffin embedded sections of human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9H9S0

Internal Search Terms :

Nanog homeobox, Nanog, Homeobox transcription factor Nanog-delta 48, Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog, FLJ40451, FLJ12581, , , ANA0837-10ug, ANA0837-20ug, ANA0837-50ug, ANA0837-100ug, ANA0837-250ug, ANA0837-500ug, ANA0837-1mg, ANA0837-010, ANA0837-020, ANA0837-050, ANA0837-100, ANA0837-250, ANA0837-500, ANA0837-01M

more info or order :

NKMAX product webpage