Human MIF antibody | NKMAX AMF0608 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MIF antibody

catalog :

AMF0608

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MIF antibody

Catalog number (SKU) :

AMF0608

NCBI :

NP_002406

Description :

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Alternative Names :

Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor),

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.;; Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155.;; Richard B, et al., (1996) FASEB J. 10:1607-1613.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human MIF (1-115 aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AMF0608 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of MIF in HeLa cells. The cell was stained with AMF0608 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{AMF0608-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: THP-1 cell lysate;;Lane 3.: HeLa cell lysate;;Lane 4.: U937 cell lysate The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {AMF0608-WB2.jpg}

Reacts With :

Human

Clone Number :

4E4

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P14174

Internal Search Terms :

MIF,AMF0608-10ug,AMF0608-20ug,AMF0608-50ug,AMF0608-100ug,AMF0608-250ug,AMF0608-500ug,AMF0608-1mg,AMF0608-10,AMF0608-20,AMF0608-50,AMF0608-100,AMF0608-250,AMF0608-500,AMF0608-1

References with this Product :

Koizumi M, et al. Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows. (J Reprod Dev. 2016){https://pubmed.ncbi.nlm.nih.gov/26853787/}

more info or order :

NKMAX product webpage