E.coli Maltose Binding Protein/MBP antibody | NKMAX AMB0710 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

E.coli Maltose Binding Protein/MBP antibody

catalog :

AMB0710

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C12

reactivity :

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

E.coli Maltose Binding Protein/MBP antibody

Catalog number (SKU) :

AMB0710

NCBI :

NP_418458.1

Description :

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

Alternative Names :

ZCSL3, MMBP, MBP, Maltose binding protein, Maltodextrin-binding protein, malJ, malE, JW3994, JJJ3, Escherichia coli MBP, ECK4026, E.coli MBP, DPH4, Cytoplasmic maltose-binding protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jonathan D et al.,(1996) Proc Natl Acad Sci USA 93:211-215.;; Weiming Y et al., (2001) EMBO J 20(3):362-371.;; Chen Z et al., (2005) Proc Natl Acad Sci USA 102(29):10200-10205.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant MBP purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MBP-tagged WLS protein(58.9kDa);;Lane 2.: MBP-tagged IRF7 protein(59.3kDa);;

Clone Number :

10C12

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

E.coli

Gene Category :

Protein Marker

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P0AEX9

Internal Search Terms :

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more info or order :

NKMAX product webpage