protein

Recombinant human Aldehyde dehydrogenase 2/ALDH2 protein

ALD0905

20 ug

55 USD

Recombinant human Aldehyde dehydrogenase 2/ALDH2 protein

18-517aa

ALD0905

NP_000681.2

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM

1mg/ml (determined by absorbance at 280nm)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1mM EDTA, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Crabb DW., et al. (1989). J Clin Invest. 83(1):314-6;; Feng Liu., et al. (2002). Plant Physiol. 130(4):1657-1674;;

> 90% by SDS-PAGE

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25℃.

SDS-PAGE, Enzyme Activity

Human

Redox Proteins

54.5 kDa (501aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25℃.

MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S

Non-Tagged

E.coli

Recombinant Protein

P05091

ALDH2, Aldehyde dehydrogenase mitochondrial, ALDH-E2, ALDHI, ALDM, Aldehyde dehydrogenase, Mitochondrial Acetaldehyde dehydrogenase 2, Aldehyde dehydrogenase 2 family, Aldehyde dehydrogenase 2 family (mitochondrial), ALDHE2, ALDH 2, ALDH class 2, ALDH E2, Liver mitochondrial ALDH, MGC1806, Mitochondrial aldehyde dehydrogenase 2, Nucleus encoded mitochondrial aldehyde dehydrogenase 2, ALD0905-10ug, ALD0905-20ug, ALD0905-50ug, ALD0905-100ug, ALD0905-250ug, ALD0905-500ug, ALD0905-1mg, ALD0905-010, ALD0905-020, ALD0905-050, ALD0905-100, ALD0905-250, ALD0905-500, ALD0905-01M

Hosoi T, et al. Flurbiprofen ameliorated obesity by attenuating leptin resistance induced by endoplasmic reticulum stress. (EMBO Mol Med. 2014){https://pubmed.ncbi.nlm.nih.gov/24421337/};;Stiti N, et al. Nicotinamide Cofactors Suppress Active-Site Labeling of Aldehyde Dehydrogenases. (ACS Chem Biol. 2016){https://pubmed.ncbi.nlm.nih.gov/26990764/}