antibody

Human IRF5 antibody

AIR0611

50 ul

170 USD

monoclonal

mouse

nonconjugated

10T1

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

Human IRF5 antibody

AIR0611

NP_116032.1

Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

interferon regulatory factor 5, irf5

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Mario B., et al, (2007) J. Virology 81(9):4473-4481.;; Ning S., et al, (2005) J. Virology 79:11671-11676.

Mouse

IgG1 kappa

Recombinant human IRF-5 (176-240aa) purified from E. coli

ELISA, WB, ICC/IF, IHC, FACS

ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with AIR0611 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with AIR0611 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{AIR0611-IF2.jpg}

The cell lysates of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Human

10T1

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Human

Hormones & Cytokines

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal Antibody

Q13568

IRF5,AIR0611-10ug,AIR0611-20ug,AIR0611-50ug,AIR0611-100ug,AIR0611-250ug,AIR0611-500ug,AIR0611-1mg,AIR0611-10,AIR0611-20,AIR0611-50,AIR0611-100,AIR0611-250,AIR0611-500,AIR0611-1

Barro M, Patton JT. Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7. (J Virol. 2007){https://pubmed.ncbi.nlm.nih.gov/17301153/};;Watanabe T, et al. Muramyl dipeptide activation of nucleotide-binding oligomerization domain 2 protects mice from experimental colitis. (J Clin Invest. 2008){https://www.ncbi.nlm.nih.gov/pubmed/18188453};;Ye L, et al. Methamphetamine enhances Hepatitis C virus replication in human hepatocytes. (J Viral Hepat. 2008){https://pubmed.ncbi.nlm.nih.gov/18307590/};;Pandey AK, et al. NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to Mycobacterium tuberculosis. (PLoS Pathog. 2009){https://www.ncbi.nlm.nih.gov/pubmed/19578435};;Polioudakis D, et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. (Nucleic Acids Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23303785/};;Steinhagen F, et al. IRF-5 and NF-κB p50 co-regulate IFN-β and IL-6 expression in TLR9-stimulated human plasmacytoid dendritic cells. (Eur J Immunol. 2013){https://pubmed.ncbi.nlm.nih.gov/23616277/};;He H, et al. Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype. (J Biol Chem. 2013){https://pubmed.ncbi.nlm.nih.gov/23878196/};;O Carroll C, et al. Identification of a unique hybrid macrophage-polarization state following recovery from lipopolysaccharide tolerance. (J Immunol. 2014){https://pubmed.ncbi.nlm.nih.gov/24337373/};;Chionh YT, et al. Protease-activated receptor 1 suppresses Helicobacter pylori gastritis via the inhibition of macrophage cytokine secretion and interferon regulatory factor 5. (Mucosal Immunol. 2015){https://pubmed.ncbi.nlm.nih.gov/24866378/};;Steinhagen F, et al. IRF5 and IRF8 modulate the CAL-1 human plasmacytoid dendritic cell line response following TLR9 ligation. (Eur J Immunol. 2016){https://pubmed.ncbi.nlm.nih.gov/26613957/};;Li D, et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. (Sci Rep. 2016){https://pubmed.ncbi.nlm.nih.gov/27481535/};;Koelzer VH, et al. Digital analysis and epigenetic regulation of the signature of rejection in colorectal cancer. (Oncoimmunology. 2017){https://pubmed.ncbi.nlm.nih.gov/28507795/};;Xiao Y, et al. An oligodeoxynucleotide with AAAG repeats significantly attenuates burn-induced systemic inflammatory responses via inhibiting interferon regulatory factor 5 pathway. (Mol Med. 2017){https://pubmed.ncbi.nlm.nih.gov/28620671/};;Chen X, et al. MicroRNA-302a suppresses influenza A virus-stimulated interferon regulatory factor-5 expression and cytokine storm induction. (J Biol Chem. 2017){https://pubmed.ncbi.nlm.nih.gov/29046356/}