Human HSP70/HSPA1A antibody | NKMAX AHS0703 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human HSP70/HSPA1A antibody

catalog :

AHS0703

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E7

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human HSP70/HSPA1A antibody

Catalog number (SKU) :

AHS0703

NCBI :

NP_005336

Description :

Hsp70 is a human heat shock protein. Hsp70 is an important part of the cell's machinery for protein folding, and help to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. Some of these are only expressed under stress conditions, while some are present in cells under normal growth conditions and are not heat-inducible. Hsp70 can be found in different cellular compartments (nuclear, cytosolic, mitochondrial, endoplasmic reticulum, etc. ), and the chaperone function of hsp70 is required for protection against stress-induced apoptosis.

Alternative Names :

Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lewis MJ, et al., (1985) EMBO J 4:3137-3143.;; Gething M-J, et al., (1992) Nature 355:33-45.;; Dick D.M, et al., (2000) Mol Cell Biol 20(19):7146-7159.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Hsp70 (1-641aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC

IF Comment And Pic :

ICC/IF analysis of HSP70 in A549 cells line. The cell was stained with AHS0703 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp70 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: 293T cell lysate;; Lane 2.: HeLa cell lysate

Reacts With :

Human

Clone Number :

4E7

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Heat Shock Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P0DMV8

Internal Search Terms :

Heat shock 70 kDa protein 1A, Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP 70, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B, AHS0703-10ug, AHS0703-20ug, AHS0703-50ug, AHS0703-100ug, AHS0703-250ug, AHS0703-500ug, AHS0703-1mg, AHS0703-010, AHS0703-020, AHS0703-050, AHS0703-100, AHS0703-250, AHS0703-500, AHS0703-01M

more info or order :

NKMAX product webpage