Human Phosphoserine phosphatase/PSPH antibody | NKMAX AHP0705 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Phosphoserine phosphatase/PSPH antibody

catalog :

AHP0705

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Phosphoserine phosphatase/PSPH antibody

Catalog number (SKU) :

AHP0705

NCBI :

NP_004568

Description :

Human phosphoserine phosphatase (HPSP), specific for D- and L- phosphoserine, has been identified in all human tissues. HPSP is a Mg (2+) -dependent phosphoserine phosphatase. The three dimensional structure of HPSP reveals the structural and functional role of the divalent cation in the active site of phosphatases. In particular, the complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important for the substrate recognition and hydrolysis.

Alternative Names :

PSPH, PSPase, PSP, Phosphoserine phosphatase Human, Phosphoserine phosphatase deficiency, Phosphoserine phosphatase, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC 3.1.3.3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Moro-Furlani, et al., (1980) Ann Hum Genet 43(4):323-3233.;; Kim HY, et al., (2002) J Biol Chem 277(48):46651-46658.;; Peeraer Y, et al., (2004) Eur J Biochem 271(16):3421-3427.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PSP (1-225aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PSP in A431 cells. The cell was stained with AHP0705 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Jurkat cell lysate;; Lane 2.: K562 cell lysate;; Lane 3.: HepG2 cell lysate;; Lane 4.: A549 cell lysate;; Lane 5.: MCF7 cell lysate;;

Reacts With :

Human

Clone Number :

3G12

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P78330

Internal Search Terms :

PSPH, Phosphoserine phosphatase Human, PSPase, PSP, EC 3.1.3.3, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, L 3 phosphoserine phosphatase, O phosphoserine phosphohydrolase, Phosphoserine phosphatase deficiency, AHP0705-10ug, AHP0705-20ug, AHP0705-50ug, AHP0705-100ug, AHP0705-250ug, AHP0705-500ug, AHP0705-1mg, AHP0705-010, AHP0705-020, AHP0705-050, AHP0705-100, AHP0705-250, AHP0705-500, AHP0705-01M

more info or order :

NKMAX product webpage