Human HMG-1/HMGB1 antibody | NKMAX AHM0915 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human HMG-1/HMGB1 antibody

catalog :

AHM0915

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J2E1

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, other

more info or order :

NKMAX product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
other; mouse; 1:100; fig 2b immunohistochemistry - paraffin section; mouse; 1:100; fig s1 western blot; mouse; 1:1000; fig 3c	Ito H, Fujita K, Tagawa K, Chen X, Homma H, Sasabe T, et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. EMBO Mol Med. 2015;7:78-101 pubmed publisher
immunohistochemistry; human	Hashizume H, Fujiyama T, Kanebayashi J, Kito Y, Hata M, Yagi H. Skin recruitment of monomyeloid precursors involves human herpesvirus-6 reactivation in drug allergy. Allergy. 2013;68:681-9 pubmed publisher

product information

Product Type :

Antibodies

Item Name :

Human HMG-1/HMGB1 antibody

Catalog number (SKU) :

AHM0915

NCBI :

NP_002119

Description :

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.

Alternative Names :

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Palumbo R J, et al., (2004) Cell Biol. Feb 2 164(3).;; Andersson U, et al., (2002) Leukoc Biol. Dec. 72(6):1084-91.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human HMGB1 (1-215aa) purified from Baculovirus

Applications :

ELISA, WB, IHC

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMGB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;; Lane 1.: K562 cell lysate;;Lane 1.: MCF7 cell lysate;;

Reacts With :

Human

Clone Number :

J2E1

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P09429

Internal Search Terms :

Sulfoglucuronyl carbohydrate binding protein, SBP-1, SBP1, SBP 1, HMGB1, HMGB 1, HMG3, HMG1, HMG 1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin, HMG 3, High mobility group box1, High mobility group (nonhistone chromosomal) protein 1, High mobility group protein 1, AHM0915-10ug, AHM0915-20ug, AHM0915-50ug, AHM0915-100ug, AHM0915-250ug, AHM0915-500ug, AHM0915-1mg, AHM0915-010, AHM0915-020, AHM0915-050, AHM0915-100, AHM0915-250, AHM0915-500, AHM0915-01M

References with this Product :

Hashizume H, et al. Skin recruitment of monomyeloid precursors involves human herpesvirus-6 reactivation in drug allergy. (Allergy. 2013){https://pubmed.ncbi.nlm.nih.gov/23573902/};;Ito H, et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. 9EMBO Mol Med. 2015){https://pubmed.ncbi.nlm.nih.gov/25510912/}

more info or order :

NKMAX product webpage