antibody

Human Hexokinase 1/2 antibody

AHK0812

50 ul

170 USD

monoclonal

mouse

nonconjugated

1A7

western blot, ELISA, immunocytochemistry, FACS

Human Hexokinase 1/2 antibody

AHK0812

NP_000180

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase-1, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, HK1-s, HK1-ta, HK1-tc, HK2, HXK1, mHk1-s, Muscle form hexokinase, Tumor isozyme

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Furuta H, et al.,(1996) Genomics 36(1):206-209.;;Jon E, et al., (2003) J Exp Biology 206:2049-2057.

Mouse

IgG1 kappa

Recombinant human Hexokinase 2 (1-917aa) purified from E. coli

ELISA, WB, ICC/IF, FACS

ICC/IF analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Hexokinase 1 recombinant protein;;Lane 2.: Hexokinase 2 recombinant protein;;Lane 3.: Hexokinase 3 recombinant protein;;Lane 4.: Hexokinase 4 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: K562 cell lysate;;Lane 2.: Jurkat cell lysate{AHK0812-WB2.jpg}

Human

1A7

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Human

Signal Transduction

Unconjugated

Monoclonal

By protein-G affinity chromatography

Monoclonal Antibody

P52789

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase1, Hexokinase-1, Hexokinase2, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HKI, HKII, HXK 1, HXK 2, HXK1, HXK-1, HXK2, HXK-2, mHk1-s, Muscle form hexokinase, tumor isozyme, AHK0812-10ug, AHK0812-20ug, AHK0812-50ug, AHK0812-100ug, AHK0812-250ug, AHK0812-500ug, AHK0812-1mg, AHK0812-010, AHK0812-020, AHK0812-050, AHK0812-100, AHK0812-250, AHK0812-500, AHK0812-01M