S. Japonicum Glutathione S-transferase/GST Antibody | NKMAX AGT0501 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

S. Japonicum Glutathione S-transferase/GST Antibody

catalog :

AGT0501

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E5

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

S. Japonicum Glutathione S-transferase/GST Antibody

Catalog number (SKU) :

AGT0501

NCBI :

AAB59203.1

Description :

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors.

Alternative Names :

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Smith, D., et al., Proc. Natl. Acad. Sci. USA, 83, 8703 (1986).;; Cartwright, G., et al., J. Immunol. Meth., 179, 31 (1995).;; Hibma, M., et al., Nucl. Ac. Res., 22, 3806 (1994).

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant Schistosoma japonicum Glutathione S transferase (GST) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of GST in HeLa cells. The cell was stained with AGT0501 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein GST (50ng,28kDa) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; The recombinant human GST-fusion protein (50ng,49kDa) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; {2004010011202255.jpg}

Clone Number :

1E5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Schistosoma japonicum

Gene Category :

Protein Marker

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P08515

Internal Search Terms :

GST,AGT0501-10ug,AGT0501-20ug,AGT0501-50ug,AGT0501-100ug,AGT0501-250ug,AGT0501-500ug,AGT0501-1mg,AGT0501-10,AGT0501-20,AGT0501-50,AGT0501-100,AGT0501-250,AGT0501-500,AGT0501-1

more info or order :

NKMAX product webpage