Human GSK-3 beta antibody | NKMAX AGK0601 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GSK-3 beta antibody

catalog :

AGK0601

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GSK-3 beta antibody

Catalog number (SKU) :

AGK0601

NCBI :

NP_002084

Description :

Glycogen synthase kinase 3beta (GSk3beta) is a serine/threonine kinase involved in insulin, growth factor and Wnt signaling. Glycogen synthase kinase 3beta (GSk3beta) is a molecular regulator of cell fate in the central nervous system and a target of the phosphatidylinositol 3-kinase (PI3k) pathway. It is an enzyme with a diverse number of actions in intracellular signaling systems, regulating neuronal plasticity, gene expression and cell survival.

Alternative Names :

glycogen synthase kinase 3 beta

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Piyajit W et al., (2002) Proc Natl Acad Sci 99(12):7951-7955.;; Rana D et al., (2003) EMBO J 22(3): 494-501.;; Patrizia D S et al., (2006) Neurobiol Aging 27(3): 413-422.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GSK3 beta (341-420aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of GSK-3 beta in HeLa cells. The cell was stained with AGK0601 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of HeLa(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSK3 beta (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

1F7

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GSK-3 beta in HeLa cells. The cell was stained with AGK0601 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P49841

Internal Search Terms :

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more info or order :

NKMAX product webpage