Human Growth Hormone 1/GH1 antibody | NKMAX AGH0401 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Growth Hormone 1/GH1 antibody

catalog :

AGH0401

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

g3H5

reactivity :

human

application :

western blot, ELISA, neutralization

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Growth Hormone 1/GH1 antibody

Catalog number (SKU) :

AGH0401

NCBI :

NP_000506

Description :

Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20kDa variant form of human growth hormone (20kDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.

Alternative Names :

GH1, Growth Hormone, GH, GH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin, GHN, hGH-N

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wickens, et al., (1978) J. Biol Chem. 253(7)2483-2495.;;Chang, et al., (1978) Nature . 275,617-624.;;Ullrich. et al., (1977) Science 196, 1313-1319.;;Tsushima, et al., (2000) Endocr J. 47S17-2.;; Horan, M. et al.,(2003) Hum. Mutat. 21(4)408-423.

Additional Info Comment and Pic :

Neutralization:Measured by its ability to neutralize Growth hormone induced proliferation in Nb2-11 rat lymphoma cells. The neutralization ED50 is less than or equal to 2ug/ml in the presence of 2.5 ng/ml recombinant protein human Growth hormone.{AGH0401-addpic.jpg}

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human growth hormone (27-217aa) purified from E. coli

Applications :

ELISA, WB, Neutralization

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human hGH protein (21-217aa);;Lane 2.: Recombinant human hGH protein (27-202aa)

Reacts With :

Human

Clone Number :

g3H5

Ab Usage :

The antibody has been tested by ELISA, Western blot and Neutralization analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Growth factors

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P01241

Internal Search Terms :

GH, GH 1, GH1, GHN, GH-N, Growth Hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin, AGH0401-10ug, AGH0401-20ug, AGH0401-50ug, AGH0401-100ug, AGH0401-250ug, AGH0401-500ug, AGH0401-1mg, AGH0401-010, AGH0401-020, AGH0401-050, AGH0401-100, AGH0401-250, AGH0401-500, AGH0401-01M

more info or order :

NKMAX product webpage