Human FLIP antibody | NKMAX AFL0926 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human FLIP antibody

catalog :

AFL0926

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8B12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human FLIP antibody

Catalog number (SKU) :

AFL0926

NCBI :

NP_003870

Description :

FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1).

Alternative Names :

CASP8 and FADD-like apoptosis regulator isoform 1, CFLAR, CASH, CASP8AP1, CLARP, MRIT, Caspase homolog, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yu JW, Shi Y., (2008) Oncogene. 27(48):6216-27.;; Du C, et al., (2005) Kidney Int. 67(4):1397-409.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human FLIP (1-480aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of FLIP in HeLa cells. The cell was stained with AFL0926 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FLIP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: MCF7 cell lysate;;Lane 4.: K562 cell lysate

Reacts With :

Human

Clone Number :

AT8B12

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O15519

Internal Search Terms :

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more info or order :

NKMAX product webpage