Human FABP1/L-FABP antibody | NKMAX AFA0908 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human FABP1/L-FABP antibody

catalog :

AFA0908

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G4

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human FABP1/L-FABP antibody

Catalog number (SKU) :

AFA0908

NCBI :

NP_001434

Description :

FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallelbeta strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.

Alternative Names :

Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Atshaves BP, et al: (2004) Mol Cell Biochem. 259(1-2), 115-29.;; Nakamura T, et al: (2005) Diabestes Care. 28(11), 2728-32.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human FABP1 (1-127aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC, FACS

IF Comment And Pic :

ICC/IF analysis of FABP1 in Hep3B cells. The cell was stained with AFA0908 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: FABP1 Recombinant protein ;;Lane 2.: HepG2 cell lysate ;;Lane 3.: Liver tissues lysate ;;

Reacts With :

Human

Clone Number :

2G4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of FABP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IHC Comment And Pic :

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human FABP1 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07148

Internal Search Terms :

FABP1, L-FABP,AFA0908-10ug,AFA0908-20ug,AFA0908-50ug,AFA0908-100ug,AFA0908-250ug,AFA0908-500ug,AFA0908-1mg,AFA0908-10,AFA0908-20,AFA0908-50,AFA0908-100,AFA0908-250,AFA0908-500,AFA0908-1

References with this Product :

Schlabritz-Loutsevitch N, et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. (J Med Primatol. 2019){https://pubmed.ncbi.nlm.nih.gov/30246873/}

more info or order :

NKMAX product webpage