Human FADD antibody | NKMAX AFA0901 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human FADD antibody

catalog :

AFA0901

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J1D2

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human FADD antibody

Catalog number (SKU) :

AFA0901

NCBI :

NP_003815

Description :

FADD (Fas-associated protein with death domain) is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. This protein is implicated in survival/proliferation and cell cycle progression. FADD functions are also regulated via cellular sublocalization, protein phosphorylation, and inhibitory molecules.

Alternative Names :

Fas-associated via death domain, GIG3, MORT1, MGC8528, Fas-associated via death domain, FADD, Fas-associated via death domain FADD protein, Fas TNFRSF6 associated via death domain, Fas (TNFRSF6) associated via death domain, Fas associated via death domain, Fas associating protein, Fas associating death domain containing protein, Fas associating protein with death domain GIG 3, Growth inhibiting gene 3 protein, H sapiens mRNA for mediator of receptor induced toxicity, Mediator of receptor induced toxicity, MORT 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lea Tourneur.,et al:(2005) Medical Immunology. 4:1.;;Tsao,C.H., et al: (2008) J. Gen. Virol. 89(PT 8), 1930-1941.;; Douglas D. Bannerman, et al. (2002). J. Clin. Invest. 109:419-425.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human FADD (1-208aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,IHC

IF Comment And Pic :

ICC/IF analysis of FADD in HeLa cells. The cell was stained with AFA0901 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates of Jurkat (30ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : Raw264.7 cell lysate;; Lane 3. : MCF7 cell lysate ;;Lane 4. : A431 cell lysate ;;Lane 5. : Ramos cell lysate ;;Lane 6. : Raji cell lysate ;;Lane 7. : Balb/3T3 cell lysate ;;{AFA0901-WB2.jpg}

Reacts With :

Human

Clone Number :

J1D2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q13158

Internal Search Terms :

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more info or order :

NKMAX product webpage