Human EphA2 antibody | NKMAX AEA0909 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human EphA2 antibody

catalog :

AEA0909

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D7

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human EphA2 antibody

Catalog number (SKU) :

AEA0909

NCBI :

NP_004422

Description :

The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.

Alternative Names :

EPH receptor A2, Ephrin type-A receptor 2, EPHA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ARCC2, CTPA, CTPP1, CTRCT6

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5(3), 149-57;; Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59(1), 58-67

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human EphA2 (559-976aa) purified from E. coli

Applications :

ELISA, WB, IHC

WB Comment and Pic :

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: EphA2 extracellular domain transfected 293T cell lysate ;;Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate

Reacts With :

Human

Clone Number :

3D7

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB){AEA0909-IHC1b.jpg}

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P29317

Internal Search Terms :

EphA2, Ephrin receptor EphA2, epoxide hydrolase 1 microsomal (xenobiotic),AEA0909-10ug,AEA0909-20ug,AEA0909-50ug,AEA0909-100ug,AEA0909-250ug,AEA0909-500ug,AEA0909-1mg,AEA0909-10,AEA0909-20,AEA0909-50,AEA0909-100,AEA0909-250,AEA0909-500,AEA0909-1

more info or order :

NKMAX product webpage