Recombinant human Adenylate kinase 2 protein | NKMAX ADK0901 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Adenylate kinase 2 protein

catalog :

ADK0901

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Adenylate kinase 2 protein

Domain :

1-239aa

Catalog number (SKU) :

ADK0901

NCBI :

NP_001616.1

Description :

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Adenylate kinase 2, Mitochondrial Adenylate kinase 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, ADK2

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM DTT, 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kohler C., et al. (1999).FEBS Lett. 447(1):10-2;; Lee Y., et al. (1998).J Biol Chem. 123(1):47-54;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 25unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

28.6 kDa (259aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 25 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P54819

Internal Search Terms :

Mitochondrial Adenylate kinase 2, EC2743, EC2.7.4.3, EC 2.7.4.3, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ATP AMP transphosphorylase 2, ATP AMP transphosphorylase, ATP AMP phosphotransferase, Ak-2, AK2, Ak 2, ADK2, ADK 2, Adenylate monophosphate kinase, Adenylate kinase2, Adenylate kinase isoenzyme 2, Adenylate kinase 2 mitochondrial isoform a, Adenylate kinase 2, ADK0901-10ug, ADK0901-20ug, ADK0901-50ug, ADK0901-100ug, ADK0901-250ug, ADK0901-500ug, ADK0901-1mg, ADK0901-010, ADK0901-020, ADK0901-050, ADK0901-100, ADK0901-250, ADK0901-500, ADK0901-01M

more info or order :

NKMAX product webpage