protein

Recombinant mouse Adiponectin (globular domain) protein

ADI3001

20 ug

55 USD

Recombinant mouse Adiponectin (globular domain) protein

111-247aa

ADI3001

NP_033735

Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30 (amino acid residues, 111-247, gAcrp30) was overexpressed in E. coli and purified by using conventional chromatography techniques.

ADIPOQ, Acdc, Acrp30, Apm1, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipocyte-specific protein

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 5mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Joachim Fruebis, et al(2001) PNAS 98(4) 2005-2010;; Das,K., et al(2001) Biochem. Biophys.Res.Commun. 280(4)1120-1129;; Yamauchi T., et al(2001) Nature Medicine 7(8) 941-946;; Berg AH, et al(2001) Nature Medicine 7(8) 947-952;; Yamauchi. T., et al(2002) Nature Medicine 8(11) 1288-1295;;

> 95% by SDS-PAGE

SDS-PAGE

Mouse

Hormones & Cytokines

16 kDa (138aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN

Non-Tagged

E.coli

Recombinant Protein

Q60994

Adiponectin (globular domain), ADIPOQ, ACDC, ACRP30, APM1, GBP28, Adiponectin globular domain, Mouse, Adipocyte, C1q and collagen domain-containing protein, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, ACRP30, Adipose most abunda, Adiponectin, 30 kDa adipocyte complement related protein, ACRP 30, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipose most abundant gene transcript 1, APM 1, GBP 28, Gelatin binding protein,ADI3001-10ug,ADI3001-20ug,ADI3001-50ug,ADI3001-100ug,ADI3001-250ug,ADI3001-500ug,ADI3001-1mg,ADI3001-10,ADI3001-20,ADI3001-50,ADI3001-100,ADI3001-250,ADI3001-500,ADI3001-1

Almer G, et al. Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. (Int J Nanomedicine. 2011){https://pubmed.ncbi.nlm.nih.gov/21753879/};;Almer G, et al. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. (Biologics. 2011){https://pubmed.ncbi.nlm.nih.gov/22022204/};;Singh AK, et al. Orally active osteoanabolic agent GTDF binds to adiponectin receptors, with a preference for AdipoR1, induces adiponectin-associated signaling, and improves metabolic health in a rodent model of diabetes. (Diabetes. 2014){https://pubmed.ncbi.nlm.nih.gov/24848063/};;Khan MP, et al. Pathophysiological Mechanism of Bone Loss in Type 2 Diabetes Involves Inverse Regulation of Osteoblast Function by PGC-1α and Skeletal Muscle Atrogenes: AdipoR1 as a Potential Target for Reversing Diabetes-Induced Osteopenia. (Diabetes. 2015){https://pubmed.ncbi.nlm.nih.gov/25633418/};;Singh AK, et al. Small molecule adiponectin receptor agonist GTDF protects against skeletal muscle atrophy. (Mol Cell Endocrinol. 2017){https://pubmed.ncbi.nlm.nih.gov/27645900/}