Human Dapper 3/DACT3 antibody | NKMAX ADA0925 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Dapper 3/DACT3 antibody

catalog :

ADA0925

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A5

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Dapper 3/DACT3 antibody

Catalog number (SKU) :

ADA0925

NCBI :

NP_659493

Description :

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.

Alternative Names :

Thymus expressed gene 3-like, Dapper antagonist of beta-catenin homolog 3 (Xenopus laevis), Arginine rich region 1, MGC15476

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jiang X, et al., (2008) Cancer Cell. 13(6):529-41.;; Fisher DA, et al., (2006) Dev Dyn. 235(9):2620-30.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human DACT3 (89-202aa) purified from E. coli

Applications :

ELISA, WB, IHC

WB Comment and Pic :

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DACT3 antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Mouse Brain tissue lysate

Reacts With :

Human

Clone Number :

2A5

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Development

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q96B18

Internal Search Terms :

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more info or order :

NKMAX product webpage