Human Cathepsin L antibody | NKMAX ACT0905 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cathepsin L antibody

catalog :

ACT0905

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT18F6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cathepsin L antibody

Catalog number (SKU) :

ACT0905

NCBI :

NP_001244901

Description :

Cathepsin L, a lysosomal endopeptidase expressed in most eukaryotic cells, is a member of the papain-like family of cysteine proteinases. Cathepsin L can be induced by many different signaling events, including growth factor, secondary messenger and tumor promoters. Cathepsin L is translated as preprocathepsin L, transferred through the Golgi apparatus as procathepsin L and then stored in lysosomes as mature cathepsin L.

Alternative Names :

Procathepsin L, Cathepsin L1, Major excreted protein, MEP, CTSL, CTSL1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zheng X., et al (2009) Am J Physiol Cell Physiol. 296(1): 65-74.;;Reinheckel T., et al (2005) J Cell Sci. 118: 3387-95.;;Ishidoh K., Kominami E. (2002) Biol chem. 383(12): 1827-31.;;Roth W., et al (2000) FASEB J. 14(13): 2075-86.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human CTSL (18-333aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CTSL in A549 cells. The cell was stained with ACT0905 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: CTSB recombinant protein ;;Lane 2.: CTSD recombinant protein ;;Lane 3.: CTSE recombinant protein ;;Lane 4.: CTSF recombinant protein ;;Lane 5.: CTSH recombinant protein ;;Lane 6.: CTSK recombinant protein ;;Lane 7.: CTSL recombinant protein ;;Lane 8.: CTSS recombinant protein ;;Lane 9.: CTSW recombinant protein ;;Lane 10.: CTSZ recombinant protein {ACT0905-WB2.jpg}

Reacts With :

Human

Clone Number :

AT18F6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CTSL in A549 cells. The cell was stained with ACT0905 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07711

Internal Search Terms :

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more info or order :

NKMAX product webpage