Human Cystatin B antibody | NKMAX ACS0820 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cystatin B antibody

catalog :

ACS0820

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2F1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cystatin B antibody

Catalog number (SKU) :

ACS0820

NCBI :

NP_000091

Description :

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to from a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes in cells. CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.

Alternative Names :

CystatinB, Cystatin B, CSTB, Stefin B, PME, CST6, CystatinB CHROW21, CPI B, CYTB, EPM1, Liver thiol proteinase inhibitor, STFB

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Turk V, et al., (1991) FEBS Lett. 285(2):213-9.;; Jarvinen M, et al., (1988) Acta Histochem. 82(1):5-18.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CSTB (1-98aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of CSTB in A549 cells. The cell was stained with ACS0820 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: A549 cell lysate (35ug);;Lane 2.: U87MG cell lysate (40ug)

Reacts With :

Human

Clone Number :

2F1

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P04080

Internal Search Terms :

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more info or order :

NKMAX product webpage