Human Cofilin 1/CFL1 antibody | NKMAX ACO0927 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cofilin 1/CFL1 antibody

catalog :

ACO0927

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1C1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cofilin 1/CFL1 antibody

Catalog number (SKU) :

ACO0927

NCBI :

NP_005498

Description :

Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Alternative Names :

18 kDa phosphoprotein(p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Mannherz H.G., et al. (2007) J Mol Biol 366(3):745-755.;; Olsen J.V., et al. (2006) Cell 127(3):635-648.;; Yang N., et al. (1998) Nature 393(6687):809-812.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Cofilin 1 (1-166aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of Cofilin1 in HeLa cells. The cell was stained with ACO0927(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: K562 cell lysate;;Lane 3.: MCF7 cell lysate;;Lane 4.: LnCap cell lysate;;Lane 5.: PC3 cell lysate

Reacts With :

Human

Clone Number :

AT1C1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P23528

Internal Search Terms :

18 kDa phosphoprotein, 18 kDa phosphoprotein(p18), 18kDa phosphoprotein(p18), CFL, CFL-1, CFL 1, CFL1, Cofilin 1, Cofilin1, Cofilin-1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18, ACO0927-10ug, ACO0927-20ug, ACO0927-50ug, ACO0927-100ug, ACO0927-250ug, ACO0927-500ug, ACO0927-1mg, ACO0927-010, ACO0927-020, ACO0927-050, ACO0927-100, ACO0927-250, ACO0927-500, ACO0927-01M

more info or order :

NKMAX product webpage