Human Clusterin/CLU antibody | NKMAX ACL0711 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Clusterin/CLU antibody

catalog :

ACL0711

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A11

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Clusterin/CLU antibody

Catalog number (SKU) :

ACL0711

NCBI :

NP_001822

Description :

Clusterin has been implicated in numerous processes including active cell death, immune regulation, cell adhesion and morphological transformation. Clusterin (previousiy named SP-40) is a glycoprotein, 80kDa comprising two distinct 40kDa polypeptide chains. It shows marked similarity, both physicochemically and in immunohistological distribution, to the single chain, 80kDa, Sprotein, an additional component of the SC5b-9 complex. Clusterin is a secreted glycoprotein of which the exact function remains a matter for speculation.

Alternative Names :

Clusterin, ApoJ, clusterin (complement lysis inhibitor SP-40,40, sulfated glycoprotein 2 testosterone-repressed prostate message 2 apolipoprotein J), SGP-2, SP-40, TRPM-2, KUB1, complement lysis inhibitor, ulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Maximino R et al., (2000) Am J Pathol 157(2):393-399.;;Ingrid C.G et al., (1998) Biochem J 331:231-237.;; Maximino R et al., (1990) J Clin Invest 85:1477-1486.;; Fabien L et al., (2006) Biochem J 395(1):223-231.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Clusterin (1-333aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC, FACS

IF Comment And Pic :

ICC/IF analysis of Clusterin in U87MG cells. The cell was stained with ACL0711 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of U87MG (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

1A11

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Clusterin in U87MG cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IHC Comment And Pic :

Paraffin embedded sections of human renal cell carcinoma tissue were incubated with anti-human Clusterin (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P10909

Internal Search Terms :

Clusterin,ACL0711-10ug,ACL0711-20ug,ACL0711-50ug,ACL0711-100ug,ACL0711-250ug,ACL0711-500ug,ACL0711-1mg,ACL0711-10,ACL0711-20,ACL0711-50,ACL0711-100,ACL0711-250,ACL0711-500,ACL0711-1

more info or order :

NKMAX product webpage