Human Cathepsin D antibody | NKMAX ACH0627 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cathepsin D antibody

catalog :

ACH0627

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G2

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Product Type :

Antibodies

Item Name :

Human Cathepsin D antibody

Catalog number (SKU) :

ACH0627

NCBI :

NP_001900

Description :

The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15. 5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D

Alternative Names :

CTSD,CPSD, CLN10, MGC2311, cathepsin D, cathepsin D (lysosomal aspartyl protease), ceroid-lipofuscinosis, neuronal 10

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tang,J. (1979) Mol. Cell. Biochem. 26, 93-109.;; Fujita, H et al., (1991) Biochem. Biophys. Res. Commun. 179, 190-196.;; Fusek, M et al., (2005) Biomed Papers 149, 43-50.;; Garcia,M et al., (1996) Stem Cells 14, 642-650.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Cathepsin D (21-412 aa) purified from E. coli

Applications :

ELISA, WB, IHC

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: Hep3B cell lysate;;Lane 3.: MDA-MB-231 cell lysate;;Lane 4.: MCF7 cell lysate;; The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : Recombinant Human CTSB;;Lane 2. : Recombinant Human CTSD;;Lane 3. : Recombinant Human CTSE;;Lane 4. : Recombinant Human CTSF;;Lane 5. : Recombinant Human CTSH;;Lane 6. : Recombinant Human CTSK;;Lane 7. : Recombinant Human CTSL;;Lane 8. : Recombinant Human CTSS;;Lane 9. : Recombinant Human CTSW;;Lane 10. : Recombinant Human CTSZ;;{ACH0627-WB2.jpg}

Reacts With :

Human

Clone Number :

4G2

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07339

Internal Search Terms :

Cathepsin D,ACH0627-10ug,ACH0627-20ug,ACH0627-50ug,ACH0627-100ug,ACH0627-250ug,ACH0627-500ug,ACH0627-1mg,ACH0627-10,ACH0627-20,ACH0627-50,ACH0627-100,ACH0627-250,ACH0627-500,ACH0627-1

References with this Product :

McNeill A, et al. Ambroxol improves lysosomal biochemistry in glucocerebrosidase mutation-linked Parkinson disease cells. Brain. 2014 May;137(Pt 5):1481-95.{https://www.ncbi.nlm.nih.gov/pubmed/24574503};;Shen C, et al. Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network. Proteomics. 2012 Jun;12(12):1917-27.{https://pubmed.ncbi.nlm.nih.gov/22623320/}

Size 2 :

50 ul

List Price 2 (USD) :

170 USD