Human 5′-Nucleotidase/CD73 antibody | NKMAX ACD0806 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human 5′-Nucleotidase/CD73 antibody

catalog :

ACD0806

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT89E9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human 5′-Nucleotidase/CD73 antibody

Catalog number (SKU) :

ACD0806

NCBI :

NP_002517

Description :

CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Alternative Names :

CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sern P.C, et al., (2006) Purinergic Signal 2(2):351-360.;; Linda F.T, et al., (2004) J Exp Med 200(11):1395-1405.;; Laura A, et al., (1997) J Cell Biol 136(2):421-431.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CD73 (27-252aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of 5'-Nucleotidase/CD73 in HeLa cells. The cell was stained with ACD0806 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 5'-Nucleotidase/CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: WiDr cell lysate;;Lane 3.: A431 cell lysate;;

Reacts With :

Human

Clone Number :

AT89E9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of 5'-Nucleotidase/CD73 in A431 cells. The cell was stained with ACD0806 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P21589

Internal Search Terms :

Purine 5-Prime-Nucleotidase, NTE, NT5E, NT5, NT, eNT, eN, ecto-5'-nucleotidase, ecto, CD73, CALJA, AI447961, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5-nucleotidase, 5'-nucleotidase, 5-NT, 5'-NT, 5' nucleotidase ecto, 5 nucleotidase ecto, 5' nucleotidase, 5 nucleotidase, 5' NT, 2210401F01Rik, ACD0806-10ug, ACD0806-20ug, ACD0806-50ug, ACD0806-100ug, ACD0806-250ug, ACD0806-500ug, ACD0806-1mg, ACD0806-010, ACD0806-020, ACD0806-050, ACD0806-100, ACD0806-250, ACD0806-500, ACD0806-01M

more info or order :

NKMAX product webpage