Human CD45 antibody | NKMAX ACD0802 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CD45 antibody

catalog :

ACD0802

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G4

reactivity :

human

application :

ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CD45 antibody

Catalog number (SKU) :

ACD0802

NCBI :

NM_002838

Description :

CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180, 000 to 220, 000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions,

Alternative Names :

protein tyrosine phosphatase, receptor type, C (PTPRC), CD45, B220, CD 45, CD45 antigen, GP180, L CA, LCA, Leukocyte common antigen, LY5, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, PTPRC, SCID due to PTPRC deficiency, T200, T200 glycoprotein,

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Evgueni K , et al., (2005) Immunogenetics 57(5):374-383;; Holmes N., (2006) Immunology 117(2):145-155;; Francois S , et al., (2004) Immunology 113(4):441-452;; Tara S , et al., (2003) Proc Natl Acad Sci U S A 100(10):5997-6002

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human CD45 (1029-1249aa) purified from E. coli

Applications :

ELISA,FACS

Reacts With :

Human

Clone Number :

3G4

Ab Usage :

The antibody has been tested by ELISA and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CD45 in Jurkat cells. The cell was stained with ACD0802 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P08575

Internal Search Terms :

CD45, protein tyrosine phosphatase, receptor type, C (PTPRC), CD45, B220, CD 45, CD45 antigen, GP180, L CA, LCA, Leukocyte common antigen, LY5, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, PTPRC, SCID due to PTPRC deficiency, T200, T200 glycoprotein,ACD0802-10ug,ACD0802-20ug,ACD0802-50ug,ACD0802-100ug,ACD0802-250ug,ACD0802-500ug,ACD0802-1mg,ACD0802-10,ACD0802-20,ACD0802-50,ACD0802-100,ACD0802-250,ACD0802-500,ACD0802-1

more info or order :

NKMAX product webpage