antibody

Human Cyclophilin B/PPIB antibody

ACB0825

50 ul

170 USD

monoclonal

mouse

nonconjugated

k2E2

western blot, ELISA, immunocytochemistry, FACS

Human Cyclophilin B/PPIB antibody

ACB0825

NP_000933

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin.

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Watashi K, et al., (2005) Mol Cell 19(1):111-112.;; Yurchenko V, et al., (2001) Biochem Biophys Res Commun 288(4):786-788.;; E.Roydon P, et al., (1991) Proc Natl Acad Sci USA 88:1903-1907.

Mouse

IgG1 kappa

Recombinant human Cyclophilin B (26-216aa) purified from E. coli

ELISA, WB, ICC/IF, FACS

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ACB0825 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2;;Lane 2.: HeLa The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: K562 cell lysate;;Lane 3.: 293T cell lysate;;Lane 4.: A549 cell lysate;;Lane 5.: MCF7 cell lysate;;Lane 6.: SK-OV-3 cell lysate;;Lane 7.: LnCap cell lysate {ACB0825-WB2.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Cyclophilin B recombinant protein;;Lane 2.: Cyclophilin A recombinant protein;;Lane 3.: Cyclophilin D recombinant protein;;Lane 4.: Cyclophilin E recombinant protein;;Lane 5.: Cyclophilin F recombinant protein;;Lane 6.: Cyclophilin G recombinant protein;;Lane 7.: Cyclophilin H recombinant protein {ACB0825-WB3.jpg}

Human

k2E2

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of Cyclophilin B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Human

Signal Transduction

Unconjugated

Monoclonal

By protein-G affinity chromatography

Monoclonal Antibody

P23284

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, ACB0825-10ug, ACB0825-20ug, ACB0825-50ug, ACB0825-100ug, ACB0825-250ug, ACB0825-500ug, ACB0825-1mg, ACB0825-010, ACB0825-020, ACB0825-050, ACB0825-100, ACB0825-250, ACB0825-500, ACB0825-01M

Choi JW, et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. (PLoS Genet. 2009){https://pubmed.ncbi.nlm.nih.gov/19997487/};;Dubnikov T, et al. PrP-containing aggresomes are cytosolic components of an ER quality control mechanism. (J Cell Sci. 2016){https://pubmed.ncbi.nlm.nih.gov/27550517/};;Mukadam AS, et al. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. (Cell Mol Life Sci. 2018){https://pubmed.ncbi.nlm.nih.gov/29368044/}