Human Crystallin alpha B/CRYAB antibody | NKMAX ACB0617 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Crystallin alpha B/CRYAB antibody

catalog :

ACB0617

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2E8.

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Crystallin alpha B/CRYAB antibody

Catalog number (SKU) :

ACB0617

NCBI :

NP_001876.1

Description :

alpha-Crystallins are composed of two gene products; alpha-Crystallin A and B, for acidic and basic, respectively. alpha-Crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-Crystallin A and B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. alpha-Crystallin B is expressed widely in many tissues and organs and occurs in many neurological diseases.

Alternative Names :

Crya2, Crya-2, Cryab, Crystallin alpha B, Alpha-crystallin B chain, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hasan, A., et al. (2002) Biochemistry 41(52) 15876-15882.;; Kamradt,M.C., et al (2002) J. Biol. Chem. 277(41) 38731-38736.;; Reddy, G.B., et al (2002) FEBS lett. 522(1-3) 59-64.;; Ito, H., et al (2002) J. Biochem. 131(4) 593-603.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Crystallin alpha B (1-175 aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of Crystallin alpha B in HeLa cells. The cell was stained with ACB0617 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) of crystallin alpha A (alphaA) and the tissue lysates (40ug) of mouse eye (ME) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human crystallin alpha B antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

2E8.

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Heat Shock Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P02511

Internal Search Terms :

AACRYA, Alpha crystallin B chain, Alpha(B) crystallin, Alpha-crystallin B chain, Crya2, Crya-2, Cryab, CRYAB (Crystallin alpha B), Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, NY REN 27 antigen, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, ACB0617-10ug, ACB0617-20ug, ACB0617-50ug, ACB0617-100ug, ACB0617-250ug, ACB0617-500ug, ACB0617-1mg, ACB0617-010, ACB0617-020, ACB0617-050, ACB0617-100, ACB0617-250, ACB0617-500, ACB0617-01M

more info or order :

NKMAX product webpage