Human Calmodulin 2/CALM2 antibody | NKMAX ACA0830 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Calmodulin 2/CALM2 antibody

catalog :

ACA0830

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J4D8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Calmodulin 2/CALM2 antibody

Catalog number (SKU) :

ACA0830

NCBI :

NP_001734

Description :

Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.

Alternative Names :

PHKD, CAMII, PHKD2, Calmodulin 2, CALM 2, Calmodulin2, CAM II, LP7057 protein, PHKD 2, Phosphorylase kinase delta, Calmodulin-2, CALM2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhang M., et al. (1998). Biochem Cell Biol. 76(2-3):313-23.;; James.P., et al. (1995). Trend Biochem Sci. 20: 38-42.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human Calmodulin (1-149aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: NIH3T3 cell lysate ;;Lane 2.: HeLa cell lysate ;;Lane 3.: Jurkat cell lysate ;;Lane 4.: A549 cell lysate ;;Lane 5.: MCF7 cell lysate ;;Lane 6.: Ramos cell lysate ;;Lane 7.: PC3 cell lysate

Reacts With :

Human

Clone Number :

J4D8

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P0DP24

Internal Search Terms :

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more info or order :

NKMAX product webpage