Human CEBP beta/CEBPB antibody | NKMAX ABP0609 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CEBP beta/CEBPB antibody

catalog :

ABP0609

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

47A1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CEBP beta/CEBPB antibody

Catalog number (SKU) :

ABP0609

NCBI :

NP_005185

Description :

CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPalpha, C/EBPbeta, C/EBPdelta, C/EBPepsilon, and C/EBPgamma. Among these proteins, C/EBPbeta is usually highly expressed in liver, intestine, lung and adipose. C/EBPbeta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.

Alternative Names :

CCAAT/enhancer-binding protein beta, Liver activator protein, LAP, liver-enriched transcriptional activator protein, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, NFIL6, Transcription factor 5, TCF-5, Interleukin 6-dependent DNA-binding protein, IL6DBP, CRP2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lekstrom-Himes J. et al. (1998) J Biol. Chem. 273, 28545-28548.;; Parkin SE. et al. (2002) J Biol. Chem. 277:23563-23572.;; Su WC. et al. (2003) J Biol. Chem. 278, 51150-51158.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human C/EBP-beta (1-271 aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of C/EBP-beta in HaLa cells. The cell was stained with ABP0609 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate

Reacts With :

Human

Clone Number :

47A1

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P17676

Internal Search Terms :

EBP-b,ABP0609-10ug,ABP0609-20ug,ABP0609-50ug,ABP0609-100ug,ABP0609-250ug,ABP0609-500ug,ABP0609-1mg,ABP0609-10,ABP0609-20,ABP0609-50,ABP0609-100,ABP0609-250,ABP0609-500,ABP0609-1

more info or order :

NKMAX product webpage