Human BHMT antibody | NKMAX ABH0708 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human BHMT antibody

catalog :

ABH0708

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human BHMT antibody

Catalog number (SKU) :

ABH0708

NCBI :

AAH12616

Description :

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.

Alternative Names :

Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Mckeever MP et al.,(1991) Clin Sci 81(4):551-556;; Norihiko F et al., (2001) Biochem J 129:313-320;; Francisco G et al., (2005) Biochem J 391:589-599;; Catherine M et al., (2005) Biochem J 392:443-448;; Janet D. S et al., (2006) Biochem J 395:363-371

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human BHMT (1-406aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of BHMT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BHMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The extracts of mouse liver(each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

3D6

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

FACS Comment and Pic :

Flow cytometry analysis of BHMT in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q93088

Internal Search Terms :

Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, ABH0708-10ug, ABH0708-20ug, ABH0708-50ug, ABH0708-100ug, ABH0708-250ug, ABH0708-500ug, ABH0708-1mg, ABH0708-010, ABH0708-020, ABH0708-050, ABH0708-100, ABH0708-250, ABH0708-500, ABH0708-01M

References with this Product :

Li S, et al. Regulation of homocysteine homeostasis through the transcriptional coactivator PGC-1alpha. (Am J Physiol Endocrinol Metab. 2009){https://pubmed.ncbi.nlm.nih.gov/19158324/}

more info or order :

NKMAX product webpage