Human APP/Protease Nexin II antibody | NKMAX AAP0836 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human APP/Protease Nexin II antibody

catalog :

AAP0836

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J4H9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human APP/Protease Nexin II antibody

Catalog number (SKU) :

AAP0836

NCBI :

NP_000475

Description :

Amyloid precursor protein (APP) is the precursor molecule whose proteolysis generates amyloid beta (AB), a 39- to 42- amino acid peptide and this amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's diseases patients. APP is an integral membrane protein that is phosphorylated in the cytoplasmic and extracellular domains. It has been reported that cell-surface APP plays a role in neurite extension of primary cultured hippocampal neurons. The large extracellular domain of APP is also reported to bind extracellular matrix molecules such as heparin, laminin, and collagen, which can mediate cell adhesion and neurite outgrowth. Abnormal regulation of the metabolism of APP may contribute to the deposition of plaques.

Alternative Names :

amyloid beta A4 protein precursor, isoform, amyloid beta (A4) precursor protein, Alzheimer disease, peptidase nexin-II

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Suzuki, T. et al., (1994) EMBO J. 13, 1114-1122.;; Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human APP (18-289aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAP0836 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse brain tissue lysate

Reacts With :

Human

Clone Number :

J4H9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of APP/Protease Nexin II in 293T cells. The cell was stained with AAP0836 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P05067

Internal Search Terms :

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more info or order :

NKMAX product webpage